ImageVerifierCode 换一换
格式:PDF , 页数:2 ,大小:93.10KB ,
资源ID:343958    下载:注册后免费下载
快捷下载
登录下载
邮箱/手机:
温馨提示:
快捷下载时,用户名和密码都是您填写的邮箱或者手机号,方便查询和重复下载(系统自动生成)。 如填写123,账号就是123,密码也是123。
特别说明:
请自助下载,系统不会自动发送文件的哦; 如果您已付费,想二次下载,请登录后访问:我的下载记录
验证码:   换一换

加入VIP,免费下载
 

温馨提示:由于个人手机设置不同,如果发现不能下载,请复制以下地址【https://www.wenkunet.com/d-343958.html】到电脑端继续下载(重复下载不扣费)。

已注册用户请登录:
账号:
密码:
验证码:   换一换
  忘记密码?
三方登录: 微信登录   QQ登录   微博登录 

下载须知

1: 本站所有资源如无特殊说明,都需要本地电脑安装OFFICE2007和PDF阅读器。
2: 试题试卷类文档,如果标题没有明确说明有答案则都视为没有答案,请知晓。
3: 文件的所有权益归上传用户所有。
4. 未经权益所有人同意不得将文件中的内容挪作商业或盈利用途。
5. 本站仅提供交流平台,并不能对任何下载内容负责。
6. 下载文件中如有侵权或不适当内容,请与我们联系,我们立即纠正。
7. 本站不保证下载资源的准确性、安全性和完整性, 同时也不承担用户因使用这些下载资源对自己和他人造成任何形式的伤害或损失。

版权提示 | 免责声明

本文(MK-8745-DataSheet-MedChemExpress.pdf)为本站会员(MedChemExpress)主动上传,文库网仅提供信息存储空间,仅对用户上传内容的表现方式做保护处理,对上载内容本身不做任何修改或编辑。 若此文所含内容侵犯了您的版权或隐私,请立即通知文库网(发送邮件至13560552955@163.com或直接QQ联系客服),我们立即给予删除!

MK-8745-DataSheet-MedChemExpress.pdf

1、MK-8745Cat. No.: HY-13819CAS No.: 885325-71-3Molecular Formula: CHClFNOSMolecular Weight: 431.91Target: Aurora KinasePathway: Cell Cycle/DNA Damage; EpigeneticsStorage: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 monthSolvent & SolubilityIn Vitro DMSO : 34 mg/mL (78.72 mM)* “ means s

2、oluble, but saturation unknown.ConcentrationSolvent Mass 1 mg 5 mg 10 mg1 mM 2.3153 mL 11.5765 mL 23.1530 mL5 mM 0.4631 mL 2.3153 mL 4.6306 mLPreparing Stock Solutions10 mM 0.2315 mL 1.1576 mL 2.3153 mLPlease refer to the solubility information to select the appropriate solvent.BIOLOGICAL ACTIVITYDe

3、scription MK-8745 is an aurora A kinase inhibitor with IC50s of 0.6 nM.IC & Target Aurora A, IC50: 0.6 nMIn Vitro MK-8745 induces apoptotic cell death in a p53-dependent manner when tested in vitro in cell lines of multiple lineages. Exposure of p53 wild-type cells to MK-8745 results in the inductio

4、n of p53 phosphorylation (ser15) and an increase in p53 protein expression1. 1 M of MK-8745 exposure for 24 h induces cell cycle arrest in all NHL cells, with variable degrees of G2/M arrest. Z138C cells are highly sensitive to MK-8745 (1 M) treatment and induces an approximate 5.5-fold increase in

5、the G2/M phase cell population by 96 h. MK-8745 treatment inhibits phosphorylation of Aurora-A in Granta 519 and Z138C cells, while Akata and JVM2 has no effect. MK-8745 specifically inhibits Aurora-A specific function. MK-8745 treatment leads to apoptotic cell death2.Product Data Sheet InhibitorsAg

6、onistsScreening Librarieswww.MedChemE1PROTOCOLCell Assay 2 A total of 10000 cells are plated per well in a 96-well plate and treated with MK-8745 for varying time points starting 24 h after plating. Cell viability is measured by the MTT assay2.MCE has not independently confirmed the accuracy of thes

7、e methods. They are for reference only.REFERENCES1. Jayasree S Nair et al. The induction of polyploidy or apoptosis by the Aurora A kinase inhibitor MK8745 is p53-dependent.2. Aparajita Chowdhury et al. A novel Aurora kinase A inhibitor MK-8745 predictsTPX2 as a therapeutic biomarker in non-Hodgkin lymphoma cell lines.Leuk Lymphoma, 2012 Mar, 53(3):462-71.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: techMedChemEAddress: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USAwww.MedChemE2

本站链接:文库   一言   我酷   合作


客服QQ:2549714901微博号:文库网官方知乎号:文库网

经营许可证编号: 粤ICP备2021046453号世界地图

文库网官网©版权所有2025营业执照举报