1、Sulfo-SMCC sodiumCat. No.: HY-D0975CAS No.: 92921-24-9Molecular Formula: CHNNaOSMolecular Weight: 436.37Target: OthersPathway: OthersStorage: 4C, protect from lightSolvent Need ultrasonic and warming)ConcentrationSolvent Mass 1 mg 5 mg 10 mg1 mM 2.2916 mL 11.4582 mL 22.9163 mL5 mM 0.4583 mL 2.2916 m
2、L 4.5833 mLPreparing Stock Solutions10 mM 0.2292 mL 1.1458 mL 2.2916 mLPlease refer to the solubility information to select the appropriate solvent.BIOLOGICAL ACTIVITYDescription Sulfo-SMCC sodium is a commonly used hetero-bifunctional crosslinker bearing N-hydroxysuccinimide (NHS) ester and maleimi
3、de groups to react with primary amines and sulfhydryl groups, respectively.In Vitro The crosslinker Sulfo-SMCC consists of a maleimide and an N-hydroxysuccinimide ester group to bind to sulfhydryl groups and primary amines, respectively. Sulfo-SMCC inhibits the end-to-end annealing of stabilized Mic
4、rotubules (MTs). MTs are treated with 250 M Sulfo-SMCC, and imaged after incubation for 0 h, 6 h, and 24 h. MTs treated with Sulfo-SMCC shows a constant mean length, independent of the incubation time1.PROTOCOLLabeled and unlabeled porcine tubulin powders are used. Sulfo-SMCC is used. MTs are polyme
5、rized to a final concentration of 2 mg/mL in 80 mM PIPES buffer (pH 6.8) containing 10 M taxol, 2 mM MgCl2, 0.5 mM EGTA, and 1 mM GTP. A mixture of rhodamine-labeled tubulin and unlabeled tubulin (1:5) is used in the time-dependent measurements. In these experiments, polymerized MTs are divided in 2
6、 groups: control and Sulfo-SMCC (250 M) Kinase Assay 1Product Data Sheet InhibitorsAgonistsScreening Librarieswww.MedChemE1treated. Both samples are imaged after diluting 1:5 and incubating for 0 h (within 45 minutes after polymerization), 6 h, and 24 h1. MCE has not independently confirmed the accu
7、racy of these methods. They are for reference only.REFERENCES1. Prabhune M, et al. Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro. PLoS One. 2016 Aug 25;11(8):e0161623.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: techMedChemEAddress: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USAwww.MedChemE2
