1、meta-iodoHoechst 33258Cat. No.: HY-15622CAS No.: 158013-42-4Molecular Formula: CHINMolecular Weight: 534.39Target: OthersPathway: OthersStorage: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 monthSolvent & SolubilityIn Vitro 10 mM in DMSOConcentrationSolvent Mass 1 mg 5 mg 10 mg1 mM 1.
2、8713 mL 9.3565 mL 18.7129 mL5 mM 0.3743 mL 1.8713 mL 3.7426 mLPreparing Stock Solutions10 mM 0.1871 mL 0.9356 mL 1.8713 mLPlease refer to the solubility information to select the appropriate solvent.BIOLOGICAL ACTIVITYHoechst stains are part of a family of blue fluorescent dyes used to stain DNA.IC5
3、0 Value:Target: These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258
4、and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510
5、-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 C for at least six months when protected from light. For long-term storage the solutions ar
6、e instead frozen at -20 C.The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably.Hoechst dyes are cell-permeable and ca
7、n bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, which means that cells survive a treatment with these compounds. Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm.in Descrip
8、tionProduct Data Sheet InhibitorsAgonistsScreening Librarieswww.MedChemE1vitro: N/A in vivo: N/AClinical trial: N/AREFERENCES1. Portugal J, Waring MJ. Assignment of DNA binding sites for 4,6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Bi
9、ophysica Acta 949 (2): 158-68.2. Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505.3. a b c “Hoechst Stains“. Invitrogren (Molecular Probes).Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: techMedChemEAddress: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USAwww.MedChemE2
