1、emfret Analytics GmbH therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Xia.G7 recogn
2、izes GPIba in Western blot analysis After separation of mouse platelet lysate by SDS -PAGE (non-reducing conditions) and transfer to PVDF membrane, the membrane was incubated with Xia.G7 (2-5 g/ml) and bound antibody was visualized with HRP -conjugated anti-rat IgG and ECL. References: 1. Berndt MC,
3、 Shen Y, Dopheide SM, et al. (2001) The vascular biology of the glycoprotein Ib-IX-V complex. Thromb Haemost. 2001 Jul;86(1):178-88. 2. Bergmeier W, Rackebrandt K, Schroder W, Zirngibl H, Nieswandt B. (2000) Structural and functional characterization of the mouse von Willebrand factor receptor GPIb-IX with novel monoclonal antibodies. Blood. 95:886-93. 3. emfret Analytics. Unpublished results . 150 kDa
