1、emfret Analytics GmbH therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. References: 1
2、. Berndt MC, Shen Y, Dopheide SM, et al. (2001) The vascular biology of the glycoprotein Ib-IX-V complex. Thromb Haemost. 2001 Jul;86(1):178-88. 2. Bergmeier W, Rackebrandt K, Schroder W, Zirngibl H, Nieswandt B. (2000) Structural and functional characterization of the mouse von Willebrand factor re
3、ceptor GPIb-IX with novel monoclonal antibodies. Blood. 95:886-93. 3. emfret Analytics. Unpublished results. Log fluorescence intensity. FL1 Mouse platelets Detection of GPIX on mouse platelets Mouse blood was diluted 1:20 and 25 l of this dilution were stained with 5 l control IgG-FITC (emfret Analytics, P190-1, black line) or Xia.B4-FITC (shaded area) for 15 min at RT and analyzed directly. Platelets were gated by FSC/SSC characteristics.
