1、 c-Src mouse mAb(PTR2316)Catalog No : YM4271Reactivity : Human, Mouse,RatApplications : WBGene Name : SRC SRC1Protein Name : c-SrcHuman Gene Id : 6714Human Swiss ProtNo : P12931Immunogen : Synthesized peptide derived from human c-SrcSpecificity : This antibody detects endogenous levels of human c-Sr
2、cFormulation : Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Source : MouseDilution : WB 1:1000-2000Purification : The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.Concentration : 1 mg/mlStorage Stability : -20C/1 year
3、Observed Band : 60Background : SRC proto-oncogene, non-receptor tyrosine kinase(SRC) Homo sapiens Thisgene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cellgrowth. The protein encoded by this gene is a t
4、yrosine-protein kinase whoseactivity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this1 / 2gene could be involved in the malignant progression of colon cancer. Twotranscript variants encoding the same protein have been found for this gene.provided by RefSeq, Jul 2008,Function :
5、catalytic activity:ATP + a protein-L-tyrosine = ADP + a protein-L-tyrosinephosphate.,PTM:Phosphorylated on Tyr-530 by c-Src kinase (CSK). Thephosphorylated form is termed pp60c-src. The phosphorylated tail interacts withthe SH2 domain thereby repressing kinase activity.,similarity:Belongs to theprot
6、ein kinase superfamily. Tyr protein kinase family. SRCsubfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2domain.,similarity:Contains 1 SH3 domain.,subunit:Interacts with DDEF1/ASAP1;via the SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1,PELP1, TG
7、FB1I1 and TOM1L2. Interacts with the cytoplasmic domain of MUC1,phosphorylates it and increases binding of MUC1 with beta-catenin. Interacts withRALGPS1; via the SH3 domain. Interacts with HEV ORF3 protein; via the SH3domain.,Products Images Various whole cell lysates (30ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Src antibody at1ug/ml. The HRP-conjugated anti-Mouse IgGantibody(Cat:RS0001) was used to detect the antibody.Predicted band size: 60kDaPowered by TCPDF (www.tcpdf.org)2 / 2
