1、 File .HEM321DATA20161028BIZBIZ2016-O28-WJ9.D Tgt Mass (EZX): 225.00 Injection Date : 28 Oct 16 1:37 pm +0800 Seq. Line : 0Sample Name : BIZ2016-O28-WJ9 Location : P2-A-06Acq. Operator : KFF(LCMS-02) Inj : 1Spec. Reported : MS Integration Inj Volume : 1 ulAcq. Method : D:Chem321METHODS1-POS-3MIN.MAn
2、alysis Method : D:CHEM321METHODS1-POS-3MIN-PROSRSS.MCatalog No : HY-D0937 Batch#22253 Easy-Access Method: 1-POS-3MIN 225.00Method Info : Mobile Phase: A: water(0.01%TFA) B:ACN(0.01%TFA)Gradient: 5% to 95%B within 1.3 minFlow Rate :1.8ml/minColumn :SunFire C18, 4.6*50mm,3.5um A-RP-186Oven Temperature
3、 : 45C min*0.5 1 1.5 2 2.5mAU0200400600*DAD1 B, Sig=214,4 Ref=off 1.0651.188 1.207 1.698 2.083min*0.5 1 1.5 2 2.5mAU0200400*DAD1 C, Sig=254,4 Ref=off 1.0651.194 1.710 2.0822.169min0.5 1 1.5 2 2.50200000400000600000MSD1 TIC, MS File ES-API, Pos, Scan, Frag: 701.0621.7022.082min0.5 1 1.5 2 2.505000010
4、0000150000Ion 226, MSD1 226,Target Mass 225 +H Positive, EIC=225.7:226.7 1.0642.083Integration Results for DAD1 B, Sig=214,4 Ref=offRetTim Width Area Height Area% MS(+)|-|-|-|-|-|-|1.06 0.02 1161.53 900.18 93.19 2251.19 0.01 1.40 0.31 0.11 225 1.21 0.01 3.94 -0.11 0.32 2251.70 0.01 19.57 -0.81 1.57
5、2112.08 0.03 60.03 33.01 4.82 226Integration Results for DAD1 C, Sig=254,4 Ref=offRetTim Width Area Height Area% MS(+)|-|-|-|-|-|-|1.06 0.02 845.21 654.56 79.06 2251.19 0.02 3.49 3.31 0.33 2251.71 0.02 89.00 77.44 8.32 2112.08 0.03 127.66 69.69 11.94 2262.17 0.02 3.72 3.30 0.35 233MS Report from Ins
6、trument: HY-LCMS-02HY-LCMS-02 10/31/2016 12:06:47 KFF(LCMS-02) Page 1 of 2Ret. Time: 1.06 m/z200 300 400 500 600 700 800 900020000400006000080000100000120000140000ES-API Positive226.1225.2Ret. Time: 1.70 m/z200 300 400 500 600 700 800 90002000400060008000100001200014000ES-API Positive227.2141.3225.2211.2Ret. Time: 2.08 m/z200 300 400 500 600 700 800 900010000200003000040000ES-API Positive233.2226.2MS Report from Instrument: HY-LCMS-02HY-LCMS-02 10/31/2016 12:06:47 KFF(LCMS-02) Page 2 of 2
